Composite

Part:BBa_K209445:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)

pEF1-SSFYFP-Rs1.3-Tuba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3088
    Illegal EcoRI site found at 5099
    Illegal PstI site found at 346
    Illegal PstI site found at 2581
    Illegal PstI site found at 2592
    Illegal PstI site found at 4716
    Illegal PstI site found at 5736
    Illegal PstI site found at 5889
    Illegal PstI site found at 6099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3088
    Illegal EcoRI site found at 5099
    Illegal PstI site found at 346
    Illegal PstI site found at 2581
    Illegal PstI site found at 2592
    Illegal PstI site found at 4716
    Illegal PstI site found at 5736
    Illegal PstI site found at 5889
    Illegal PstI site found at 6099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3088
    Illegal EcoRI site found at 5099
    Illegal BglII site found at 128
    Illegal BglII site found at 5000
    Illegal BglII site found at 5251
    Illegal BamHI site found at 1952
    Illegal BamHI site found at 2166
    Illegal BamHI site found at 2289
    Illegal BamHI site found at 4366
    Illegal BamHI site found at 6910
    Illegal XhoI site found at 1479
    Illegal XhoI site found at 2515
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3088
    Illegal EcoRI site found at 5099
    Illegal PstI site found at 346
    Illegal PstI site found at 2581
    Illegal PstI site found at 2592
    Illegal PstI site found at 4716
    Illegal PstI site found at 5736
    Illegal PstI site found at 5889
    Illegal PstI site found at 6099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2505
    Illegal EcoRI site found at 3088
    Illegal EcoRI site found at 5099
    Illegal PstI site found at 346
    Illegal PstI site found at 2581
    Illegal PstI site found at 2592
    Illegal PstI site found at 4716
    Illegal PstI site found at 5736
    Illegal PstI site found at 5889
    Illegal PstI site found at 6099
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 2548
    Illegal BsaI.rc site found at 2788
    Illegal BsaI.rc site found at 3836
    Illegal BsaI.rc site found at 5413
    Illegal SapI site found at 5558
    Illegal SapI site found at 6152
    Illegal SapI site found at 6586
    Illegal SapI.rc site found at 3545
    Illegal SapI.rc site found at 4322
    Illegal SapI.rc site found at 4413
    Illegal SapI.rc site found at 6108


Design Notes

Cloned into pEF5/FRT/V5-DEST.

Source

References